• Chintankumar Luhar Student
  • Parth Desai
  • Archana Mankad
  • Himanshu Pandya



microscale legumes, protein estimation, protein contents, chick pea, alfa alfa, black gram, pea, growth media


In today’s world, people are focusing more towards a healthy lifestyle. One of the most important elements for the human body is Protein. A majority part of earth’s ecosystem comprises of protein. This study presents the estimation of protein conducted in four different legume species namely, Chick pea, Alfa alfa, Black gram, and Pea in different growth media such as, Chemical fertiliser, Goat manure and Cow-dung manure. Samples of all the species were randomly selected from all the sets of growth media for protein estimation through spectrophotometry and compared it with standard BSA. The experiment showed high protein content in organic fertilisers such as Goat manure and Cow-dung manure. The growth medium of Chemical fertiliser showed poor protein content in all the samples.


Allegretta, I., Gattullo, C. E., Renna, M., Paradiso, V. M., & Terzano, R. (2019). Rapid multi-element characterization of microgreens via total-reflection X-ray fluorescence (TXRF) spectrometry. Food chemistry, 296, 86-93.

Aloo, S. O., Ofosu, F. K., Kilonzi, S. M., Shabbir, U., & Oh, D. H. (2021). Edible plant sprouts: Health benefits, trends, and opportunities for novel exploration. Nutrients, 13(8), 2882.

Barbosa, H., Slater, N. K., & Marcos, J. C. (2009). Protein quantification in the presence of poly (ethylene glycol) and dextran using the Bradford method. Analytical biochemistry, 395(1), 108-110.

Butkutė, B., Taujenis, L., & Norkevičienė, E. (2018). Small-seeded legumes as a novel food source. Variation of nutritional, mineral and phytochemical profiles in the chain: raw seeds-sprouted seeds-microgreens. Molecules, 24(1), 133.

Ebert, A. W. (2022). Sprouts and microgreens—novel food sources for healthy diets. Plants, 11(4), 571.

Eswaranpillai, Uma, Murugesan, Priyadharsini, Karuppiah, Ponmurugan. (2023). Assess the impact of cultivation substrates for growing sprouts and microgreens of selected four legumes and two grains and evaluation of its nutritional properties. Plant Science Today. 10.14719/pst.2058.

Galieni, A., Falcinelli, B., Stagnari, F., Datti, A., & Benincasa, P. (2020). Sprouts and microgreens: Trends, opportunities, and horizons for novel research. Agronomy, 10(9), 1424.

Gong, X. J., Qin, L., Liu, F., Liu, D. N., Ma, W. W., Zhang, T., ... & Luo, F. (2020). Effects of organic manure on soil nutrient content: A review. Ying Yong Sheng tai xue bao= The Journal of Applied Ecology, 31(4), 1403-1416.

Kalita, P. (2022). Production of microgreens in different soil conditions and their nutraceutical analysis. Research Journal of Biotechnology. 17. 86-90.

Massah, J., & Azadegan, B. (2016). Effect of Chemical Fertilizers on Soil Compaction and Degradation. Agricultural Mechanization in Asia, Africa and Latin America, 47(1), 44-50.

Pahalvi, H. N., Rafiya, L., Rashid, S., Nisar, B., & Kamili, A. N. (2021). Chemical fertilizers and their impact on soil health. Microbiota and Biofertilizers, Vol 2: Ecofriendly Tools for Reclamation of Degraded Soil Environs, 1-20. DOI: 10.1007/978-3-030-61010-4_1

Prasad, H., Sajwan, P., Kumari, M., & Solanki, S. P. S. (2017). Effect of organic manures and biofertilizer on plant growth, yield and quality of horticultural crop: A review. International Journal of Chemical Studies, 5(1), 217-221.

Reeve, J. R., Hoagland, L. A., Villalba, J. J., Carr, P. M., Atucha, A., Cambardella, C., ... & Delate, K. (2016). Organic farming, soil health, and food quality: considering possible links. Advances in agronomy, 137, 319-367.

Renna, M., & Paradiso, V. M. (2020). Ongoing research on microgreens: nutritional properties, shelf-life, sustainable production, innovative growing and processing approaches. Foods, 9(6), 826.

Roba, T. B. (2018). Review on: The effect of mixing organic and inorganic fertilizer on productivity and soil fertility. Open Access Library Journal, 5(06), 1.

Savci, S. (2012). An agricultural pollutant: chemical fertilizer. International Journal of Environmental Science and Development, 3(1), 73.

Sharma, S., Dhingra, P., & Koranne, S. (2020). Microgreens: Exciting new food for 21st Century. Ecology, Environment and Conservation, 26, 2020.

Smýkal, P., Aubert, G., Burstin, J., Coyne, C. J., Ellis, N. T., Flavell, A. J., & Warkentin, T. D. (2012). Pea (Pisum sativum L.) in the genomic era. Agronomy, 2(2), 74-115.

Sönmez I, Kaplan M, Sönmez S (2007) An investigation of seasonal changes in nitrate contents of soils and irrigation waters in greenhouses located in Antalya-Demre region. Asian J Chem, 19(7):5639–5646

Suhag, M. (2016). Potential of biofertilizers to replace chemical fertilizers. International Advanced Research Journal in Science, Engineering and Technology, 3(5), 163-167.

Wu, G. (2016). Dietary protein intake and human health. Food & function, 7(3), 1251-1265.




How to Cite

Luhar, C., Desai, P., Mankad, A., & Pandya, H. (2023). ROLE OF DIFFERENT GROWTH MEDIA FOR THE ASSESSMENT OF PROTEIN CONTENT IN SELECTED MICROSCALE LEGUMES. International Association of Biologicals and Computational Digest, 2(1), 32–38.




Most read articles by the same author(s)

1 2 3 4 5 > >>