EFFECT OF SALT STRESS ON SEED GERMINATION, PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF VIGNA RADIATA AND VIGNA MUNGO
DOI:
https://doi.org/10.56588/iabcd.v2i1.122Keywords:
Salt stress, Seed Germination, Protein, Starch, Reducing Sugar, Vigna radiata, Vigna mungoAbstract
The response of two bean cultivars – Vigna radiata and Vigna mungo against four different salinity levels namely 0, 50, 100 and 150 mM NaCl were studied at germination and early seedling stage. This investigation was performed as laboratory experiment under completely randomized design (CRD) with three replications for each salinity level. The effect of salt stress on various Germination, Physiological and Biochemical parameters was observed. The results indicated the adverse effect of salt stress on all the studied parameters in both the cultivars. The maximum adverse effect was seen on the seeds under 150 mM NaCl treatment. The results also concluded that V. radiata is comparatively more tolerant to salt stress than V. mungo.
References
Abdul‐Baki, A. A., & Anderson, J. D. (1973). Vigor determination in soybean seed by multiple criteria 1. Crop science, 13(6), 630-633.
Al-Ansari, F. M. (2003). Salinity tolerance during germination in two arid-land varieties of wheat (Triticum aestivum L.). Seed science and technology, 31(3), 597-603.
Alqurainy, F. (2007). Responses of bean and pea to vitamin C under salinity stress. Research Journal of Agriculture and Biological Sciences, 3(6), 714-722.
Arulbalachandran, D., Ganesh, K. S., & Subramani, A. (2009). Changes in metabolites and antioxidant enzyme activity of three Vigna species induced by NaCl stress. American-Eurasian Journal of Agronomy, 2(2), 109-116.
Ashraf, M. F. M. R., & Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and experimental botany, 59(2), 206-216.
Atta, K., Pal, A. K., & Jana, K. (2021). Effects of salinity, drought and heavy metal stress during seed germination stage in ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi]. Plant Physiology Reports, 26, 109-115.
Bartels, D., & Sunkar, R. (2005). Drought and salt tolerance in plants. Critical reviews in plant sciences, 24(1), 23-58.
Bohnert, H. J., & Jensen, R. G. (1996). Metabolic engineering for increased salt tolerance–the next step. Functional Plant Biology, 23(5), 661-667.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254.
Carpýcý, E. B., Celýk, N., & Bayram, G. (2009). Effects of salt stress on germination of some maize (Zea mays L.) cultivars. African Journal of Biotechnology, 8(19), 4918-4922.
Chinoy, J. J. (1939). A new colorimetric method for the determination of starch applied to soluble starch, natural starches, and flour. Mikrochemie vereinigt mit Mikrochimica acta, 26, 132-142.
Cokkizgin, A. (2012). Salinity stress in common bean (Phaseolus vulgaris L.) seed germination. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(1), 177-182.
Cokkizgin, A., & Cokkizgin, H. (2010). Effects of lead (PbCl2) stress on germination of lentil (Lens culinaris Medic.) lines. African Journal of Biotechnology, 9(50), 8608-8612.
Dubey, R. S. (1999). Protein synthesis by plants under stressful conditions. Handbook of plant and crop stress, 2, 365-397.
El-Keblawy, A. (2004). Salinity effects on seed germination of the common desert range grass, Panicum turgidum. Seed Science and Technology, 32(3), 873-878.
Ellis, R. H., & Roberts, E. H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology (Netherlands), 9(2), 373-409.
Farsiani, A., & Ghobadi, M. E. (2009). Effects of PEG and NaCl stress on two cultivars of corn (Zea mays L.) at germination and early seedling stages. International Journal of Agricultural and Biosystems Engineering, 3(9), 442-445.
Foolad, M. R., & Lin, G. Y. (1997). Genetic potential for salt tolerance during germination in Lycopersicon species. HortScience, 32(2), 296-300.
Gholamin, R., Khayatnezhad, M., Jamaati-e-Somarin, S., & Zabihi-e-Mahmoodabad, R. (2010). Effects of polyethylene glycol and NaCl stress on two cultivars of wheat (Triticum durum) at germination and early seeding stages. Am Eurasian J Agric Environ Sci, 9(1), 86-90.
Ghoulam, C., & Fares, K. (2001). Effect of salinity on seed germination and early seedling growth of sugar beet (Beta vulgaris L.). Seed science and technology, 29(2), 357-364.
Jaleel, C. A., Gopi, R., Kishorekumar, A., Manivannan, P., Sankar, B., & Panneerselvam, R. (2008). Interactive effects of triadimefon and salt stress on antioxidative status and ajmalicine accumulation in Catharanthus roseus. Acta Physiologiae Plantarum, 30, 287-292.
Kaya, M. D., Okçu, G., Atak, M., Cıkılı, Y., & Kolsarıcı, Ö. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European journal of agronomy, 24(4), 291-295.
Kayani, S. A., & Rahman, M. (1987). Salt tolerance in corn (zea-mays-l) at the germination stage. Pakistan Journal of Botany, 19(1), 9-15.
Kaymakanova, M. (2009). Effect of salinity on germination and seed physiology in bean (Phaseolus vulgaris L.). Biotechnology & Biotechnological Equipment, 23(sup1), 326-329.
Khayatnezhad, M., & Gholamin, R. (2011). Effects of salt stress levels on five maize (Zea mays L.) cultivars at germination stage. African Journal of Biotechnology, 10(60), 12909-12915.
Khedr, A. H. A., Abbas, M. A., Wahid, A. A. A., Quick, W. P., & Abogadallah, G. M. (2003). Proline induces the expression of salt‐stress‐responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt‐stress. Journal of experimental botany, 54(392), 2553-2562.
Khodadad, M. (2011). An evaluation of safflower genotypes (Carthamus tinctorius L.), seed germination and seedling characters in salt stress conditions. African Journal of Agricultural Research, 6(7), 1667-1672.
Khodarahmpour, Z., Ifar, M., & Motamedi, M. (2012). Effects of NaCl salinity on maize (Zea mays L.) at germination and early seedling stage. African Journal of Biotechnology, 11(2), 298-304.
Khosravinejad, F., Heydari, R., & Farboodnia, T. (2009). Effect of salinity on organic solutes contents in barley. Pakistan Journal of Biological Sciences: PJBS, 12(2), 158-162.
Kim, H. J., Feng, H., Kushad, M. M., & Fan, X. (2006). Effects of ultrasound, irradiation, and acidic electrolyzed water on germination of alfalfa and broccoli seeds and Escherichia coli O157: H7. Journal of food science, 71(6), M168-M173.
Llanes, A., Reinoso, H., & Luna, V. (2005). Germination and early growth of Prosopis strombulifera seedlings in different saline solutions. World Journal of Agricultural Sciences, 1(2), 120-128.
Moud, A. M., & Maghsoudi, K. (2008). Salt stress effects on respiration and growth of germinated seeds of different wheat (Triticum aestivum L.) cultivars. World J. Agric. Sci, 4(3), 351-358.
Munns, R., & Termaat, A. (1986). Whole-plant responses to salinity. Functional Plant Biology, 13(1), 143-160.
Parida, A., Das, A. B., & Das, P. (2002). NaCl stress causes changes in photosynthetic pigments, proteins, and other metabolic components in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures. Journal of Plant Biology, 45, 28-36.
Pujol, J. A., Calvo, J. F., & Ramirez-Diaz, L. (2000). Recovery of germination from different osmotic conditions by four halophytes from southeastern Spain. Annals of Botany, 85(2), 279-286.
Rogers, M. E., Noble, C. L., Halloran, G. M., & Nicolas, M. E. (1995). The effect of NaCl on the germination and early seedling growth of white clover (Trifolium repens L.) populations selected for high and low salinity tolerance. Seed Science and Technology (Switzerland), 23(2), 277-287.
Saboora, A., Kiarostami, K., Behroozbayati, F., & Hajihashemi, S. (2006). Salinity (NaCl) tolerance of wheat genotypes at germination and early seedling growth. Pak. J. Biol. Sci, 9(11), 2009-2021.
Sharma, A. D., Thakur, M., Rana, M., & Singh, K. (2004). Effect of plant growth hormones and abiotic stresses on germination, growth and phosphatase activities in Sorghum bicolor (L.) Moench seeds. African Journal of Biotechnology, 3(6), 308-312.
Sidari, M., Muscolo, A., Anastasi, U., Preiti, G., & Santonoceto, C. (2007). Response of four genotypes of lentil to salt stress conditions. Seed Science and technology, 35(2), 497-503.
Somogyi, M. (1952). Notes on sugar determination. Journal of biological chemistry, 195, 19-23.
Stivsev, M. V., Ponnamoreva, S., & Kuznestova, E. A. (1973). Effect of salinization and herbicides on chlorophyllase activity in tomato leaves. Fiziol. Rast, 20, 62-65.
Taffouo, V. D., Kouamou, J. K., Ngalangue, L. T., Ndjeudji, B. A. N., & Akoa, A. (2009). Effects of salinity stress on growth, ions partitioning and yield of some cowpea (Vigna unguiculata L. Walp.) cultivars. International Journal of Botany, 5(2), 135-143.
Tunçtürk, M., Tunçtürk, R., Yildirim, B., & Çiftçi, V. (2011). Effect of salinity stress on plant fresh weight and nutrient composition of some Canola (Brassica napus L.) cultivars. African Journal of Biotechnology, 10(10), 1827-1832.
Ullah, H., Scappini, E. L., Moon, A. F., Williams, L. V., Armstrong, D. L., & Pedersen, L. C. (2008). Structure of a signal transduction regulator, RACK1, from Arabidopsis thaliana. Protein Science, 17(10), 1771-1780.
Wang, K., Liu, Y., Dong, K., Dong, J., Kang, J., Yang, Q., Zhou, H., Sun, Y. (2011). The effect of NaCl on proline metabolism in Saussurea amara seedlings. African Journal of Biotechnology, 10(15), 2886-2893.
Wu, C., Wang, Q., Xie, B., Wang, Z., Cui, J., & Hu, T. (2011). Effects of drought and salt stress on seed germination of three leguminous species. African Journal of Biotechnology, 10(78), 17954-17961.